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Brain slices were obtained from E13.5 Semando/o and Sema3E+/+ mice and CH from E12.5 Actin-eGFP mouse embryos.
Surprisingly, here we found normal LTP in hippocampal brain slices of Ras-GRF2 knockout mice when single-cell recordings of CA1 pyramidal neurons in these brain slices were obtained after low frequency synaptic stimulation paired with postsynaptic depolarization (LFS pairing) (Fig. 1).
Acute brain slices were obtained from mice aged post-natal day 30 128 using standard techniques.
Brain slices were obtained from mature (> 1 month postnatal) male C57BL/6J mice, in accordance with the UK Animals (Scientific Procedures) Act 1986.
For this purpose, 2-mm-thick brain slices were obtained, which were evaluated by computer-based infarct volumetry using ImageJ software.
One millimeter-thick fresh brain slices were obtained using a mouse brain matrix and 1.2 mm diameter punches were sampled from the GFP-positive area under a fluorescent microscope and were quickly frozen in dry ice and stored at −80°C.
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Brain cortex slices were obtained from E15 mice.
Anatomical images covering whole brain with 176 sagittal slices were obtained after the functional runs using an MP-RAGE sequence with a voxel resolution of 1×1×1 mm3 (TR = 2.3 s, TI = 1.1 s, TE = 2.92 ms).
From 11 C9orf72 G4C2 expansion carriers, formalin-fixed brain was available for immunohistochemistry. Five micrometer slices were obtained from frontal cortex, temporal neocortex, hippocampus, area striata, neostriatum, mesencephalon, pons, and cerebellum.
Briefly, slices were obtained from P0 hamster brains.
25 slices were obtained to cover the whole brain.
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