Sentence examples for brain slices were fixed from inspiring English sources

Brain slices were fixed in 4% PFA at 4 °C o/n with shaking and washed in PBS for 30 mins at room temperature (RT).

After recording, brain slices were fixed with 2% paraformaldehyde and 1% glutaraldehyde and histochemically stained.

Brain slices were fixed in 4% paraformaldehye and permeabilized with ice cold methanol at −20°C for 5 minutes and then processed as above for cofilin.

The brain slices were fixed in 4% paraformaldehyde/PBS (10 mM Na-phosphate and 140 mM NaCl, pH 7.4) overnight at 4°C.

Brain slices were fixed with 10%% formaldehyde for 10 min and washed twice with PBS.

After 10 days in culture, tumor-implanted brain slices were fixed and stained for laminin.

The brains were cut in 12-μm coronal sections using a cryostat (CM3050S, Leica, Heidelberg, Germany), and the slices were fixed with 4% paraformaldehyde and stained with a monoclonal antibody against phosphorylated NF-H, MAP2, synaptophysin or MBP.

Subsequently, slices were fixed overnight in 2% paraformaldehyde at 4°C.

Immediately after incubation, slices were fixed in 4% buffered formaldehyde.

Slices were fixed, permeabilized, washed, and blocked as outlined above.

Tissue slices were fixed in 10% phosphate-buffered formalin.