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Transverse brain slices were dissected in ice cold, oxygenated (95% O2/5% CO2) artificial cerebrospinal fluid (aCSF) containing (mM): NaCl (125), KCL (2.5), KH2PO4 (1.25), MgSO4 (1.2), CaCl2 (2), NaHCO3 (25), and dextrose (10).
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A second blade was placed 1 mm (Bregma −1.10) caudal from the first, and a 1 mm thick brain slice was dissected.
The PT region slices were dissected using a surgical knife.
Hippocampal neurons and organotypic slices were dissected from the same mice.
In one dissection experiment 4 pups were dissected and all brain slices were randomly distributed on all 6-wells.
At 5 weeks post injection (5wpi), animals were killed and dissected brain slices were used for morphological and proliferative analysis by confocal microscopy.
Tissue blocks from the substantia nigra, cerebellum, and brain stem were dissected, and coronal sections were sliced at 60 μm with a Vibroslicer (VT1000S; Leica Microsystems, Vienna, Austria).
Brain regions were dissected from left hemisphere.
Brain regions were dissected and luciferase activity was measured.
Individual brain regions were dissected by a trained neuropathologist.
The anterior and posterior brain pieces were dissected.
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