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Surface radioactivity (counts per minute per mm) was measured and averaged from three replicate brain slices from each rat using Beta-vision plus software (Biospace Labs).
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LFPs were recorded in brain slices from adult mice (>P100).
Because this nanobody was previously untested, brain slices from WT animals were incubated in the nanobody to determine specificity.
We will do dissections, explore physical anatomical models, and observe microscopic structures within preserved brain slices from a variety of mammalian species.
By imaging hippocampal neurons or brain slices from MCP × MBS mice, the dynamics of mRNA, such as transcription, transport, and localization, can be studied at single mRNA resolution.
Organotypic cultures made from brain slices from specific brain regions that can be kept in culture for several weeks after injecting molecules or cells represent a remarkable tool to address these issues.
Dense Aβ aggregates (Congo red and ThT-positive) were obtained inside the collagen matrix with a homogeneous distribution and dimensions similar to those observed in post-mortem brain slices from Alzheimer's patients.
We demonstrate the use of a miniaturized electrochemical cytochrome c (Cyt c) biosensor for real-time measurements and quantitative assessment of superoxide production and inactivation by natural and engineered antioxidants in acutely prepared brain slices from mice.
To do so, we assessed excitatory synaptic function at CA3 to CA1 hippocampal synapses using extracellular field potential recordings in acute brain slices from mice fed 3 weeks with SD, CD or KD.
In acute brain slices from defeated saline-treated mice, VTA DA neurons exhibited increased excitability to current injection and higher AMPA-R/NMDA-R ratio when compared with undefeated mice (Fig. 1f, g).
Brain slices from control rats and from rats pretreated with GBR12909, fluoxetine, or nisoxetine.
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