Fresh frozen coronal, 30- μm-thick cryostat brain serial sections were collected from −1.3 to −2.2 bregma (total thickness of the examined tissue was 1500 μm).
The protection from ischemia was not confined to heart, since infarct volume, assessed in triphenyl tetrazolium chloride (TTC -stained serial secTTC -stainedrainserialysectionsmiddle cerebral artery occlusion (MCAo) (Colak et al., 2011), was signifromntly reduced in Opa1 tg mice.
Eight serial sections from each brain were cut at 2-mm intervals from the frontal pole using a rat brain matrix (Harvard Apparatus, Holliston, MA, USA).
Briefly, serial sections from each brain were stained for MAP2 and scored by an observer blinded to the treatment of the animals.
Serial sections from adult brains of the same mouse strains were exposed to drops of CPP-Cre conjugates.
In Figure 8, collagen IV immunostaining is shown on a set of serial sections from a control brain that are parallel to those illustrated for the blast-exposed animal in Figure 7.
Ten micrometer serial sections from the cryopreserved brains and spinal cords were stained with Luxol Fast blue, Hematoxylin-Eosin-Safran, anti-CD3, anti-CD68, and anti-HLA-DR (antibodies description in Table S1).
For each gene, an independently synthesized probe was hybridized on consecutive serial sections from the same brains over the span of 4 days, which maximizes comparability over time while minimizing other biological variability, including differential hapten incorporation in riboprobes, and batch reagent preparation variability.
On serial sections from one individual mouse brain we could see that SOX2 was also co-expressed with OLIG2, which is a surrogate marker for brain tumor cells [ 41, 42].
Serial sections from the paraffin-embedded brain tissue samples were assessed for neuropathology blind to clinical status according to the CERAD protocol [ 2, 3] and Braak stage [ 4, 5].
