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Brain segments were obtained from control and TBI mice, embedded in tissue freezing medium (TBS, Fisher, Pittsburg, PA, USA), frozen, cut into 30-A-m-thick sections and placed on glass slides.
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Furthermore, the training segments and testing segments were obtained from the above prepared 170 segments.
Then, each fraction was divided successively by 2 until 8 segments were obtained.
From the 2007 samples, 6 complete S segments, 1 complete M segment, and 1 complete L segment were amplified and sequenced; 7 S, 4 M, and 3 L segments were obtained from the 2010 samples.
Thus, a set of segments are obtained in a hierarchical manner.
The Leu-zipper segment was obtained by a PCR reaction from yeast cDNA library [69].
Whole brain CBV measures were obtained by first segmenting the brain using the brain extraction tool (BET) then calculating both the mean and standard deviation of CBV throughout the entire brain mask.
The colors of brain structure were obtained from the ABA, where similar colors represent related brain structures.
Virus-containing brain homogenates were obtained as follows.
Brain microvessels were obtained as described previously [18].
Human brain tissues were obtained from The Netherlands Brain Bank, Netherlands Institute for Neuroscience, Amsterdam.
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