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Meanwhile, events in the brain segment of the itch pathway are also newly unfolding.
The mean rCBF values and mean R/(R + L) ratios for each brain segment were calculated separately for the patients and the control subjects.
The two halves of the germ band are never in contact along the midline of the embryo, only the most anterior somites that will form the brain segment will be joined anteriorly at the stomodeum.
The mean rCBF values and the mean R/(R + L) ratios for each brain segment and the mCBF values for global and for right and left hemispheres were calculated separately for the non-depression and the depression group.
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The calculated field distributions were projected on the surface map of the brain, segmented from the diagnostic MRI.
The loss of this segment appeared to be caused by changes in the expression of homeobox genes (the genes that determine the identity of brain segments).
As an index of brain perfusion asymmetry, the right to right + left ratio [R/ R + L) ratio] was calculated for the 12 brain segments in each subject.
These 636 ROIs are categorized into 12 brain segments on the 3DSRT template: callosomarginal, precentral, central, parietal, angular, temporal, posterior cerebral, pericallosal, lenticular nucleus, thalamus, hippocampal, and cerebellar segments.
Brain images were entered into the voxel-based morphometry analysis using SPM5 software (http://www.fil.ion.ucl.ac.uk/spm) and the DARTEL toolbox, and grey and white matter brain segments were derived as previously described (Rohrer et al., 2010 d, e ).
Brain segments were obtained from control and TBI mice, embedded in tissue freezing medium (TBS, Fisher, Pittsburg, PA, USA), frozen, cut into 30-A-m-thick sections and placed on glass slides.
As an index of brain perfusion asymmetry, the right to right + left ratio [R/(R + L) ratio] was calculated for the 12 brain segments in each subject as follows: R/(R + L) ratio = rCBF values for the concerned segment on the right side × 100/sum of the rCBF values for the corresponding segments on the right and left sides.
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