Sentence examples for brain sections were washed from inspiring English sources

Briefly, after 24 hr of fixation, tissues were sectioned, and brain sections were washed in distilled water for several hours.

Brain sections were washed in 1% H2O2 in 50% alcohol for 20 minutes to abolish endogenous peroxidase activity.

Brain sections were washed with PBS+0.1% Triton X-100 (4–5 times, 5-minute incubations) at RT (mild shaking).

Brain sections were washed in PBS, mounted onto glass slides and cover slipped using Vectashield (Vector labs).

Briefly, mouse brain sections were washed with Tris buffer and stained for 10 min with a solution of 0.5% Thioflavin S in 50% ethanol, followed by 50% ethanol and Tris buffer, then dried and covered using Vectashield (Vector Labs, CA).

Brain sections were washed in PBS and treated with 0.2% Triton X-100, 4% bovine serum albumin (both purchased from Sigma) in PBS for 2 h, to non-specific protein binding and to permeabilize membranes.

Before appropriate secondary antibody application (rat adsorbed anti-mouse secondary antibodies were used for rat brain staining with mouse monoclonal antibodies), sections were washed twice in PBS for 5 min and then placed in secondary antibody in 5% (v/v) normal serum/PBS containing 0.15% Triton X-100 for 18 36 h at 4°C with agitation.

Brain tissue sections incubated with scFvs were washed and incubated for 3 h with anti-His6 antibodies (0.3 ug/ml, Roche) at RT. Finally, all sections were washed and incubated for 1.5 h with anti-mouse HRP-labelled antibodies (diluted 1∶1000, Jackson ImmunoResearch) at RT.

Sections were washed with distilled H2O, and air-dried.

Sections were washed, permeabilized, and stained in the following manner.

Sections were washed, decolorized, dehydrated, air-dried and cover-slipped.