Sentence examples for brain sections were treated from inspiring English sources

After 3 washes with PBS, normal brain sections were treated with NeuroTrace Fluorescent Nissl Stain (Invitrogen), a neuronal cell marker.

Rehydrated brain sections were treated with 1.5% hydrogen peroxide in methanol to inactivate endogenous peroxidase and subjected to pretreatment as described above for immunofluorescent studies.

Brain sections were treated with 2 % H2O2 in 60 % methanol for 1 h, to abolish endogenous peroxidase activity.

Brain sections were treated with DEPC to covalently modify endogenous histidyl residues and abolish their capacity to bind to nickel.

The brain sections were treated with 99% formic acid for 1 minute and then in a pressure cooker for 20 minutes.

For double immunofluorescence labeling, coronal brain sections were treated with citrate buffer, pH 6.0, for 15 min for epitope retrieval and were then incubated for 30 min with 0.3% sudan black solution to quench autofluorescence.

Following 2 h wash with PBS, both normal brain and GBM sections were treated with 1 mM CuSO4 in 50 mM NH4OAc (pH 5.0) for 90 min at room temperature to reduce lipofuscin autofluorescence [47].

Control sections were treated with preimmune serum.

Briefly, PFA-perfused brain sections were microwave treated in 0.1 M citrate buffer for 1 min and washed twice in PBS.

Brain sections were further treated with 60% methanol, 2%H2O22 for 1 h prior to incubation with the primary antibodies at 4°C overnight in a humid chamber.

Brain sections were washed in PBS and treated with 0.2% Triton X-100, 4% bovine serum albumin (both purchased from Sigma) in PBS for 2 h, to non-specific protein binding and to permeabilize membranes.