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The brain sections were stored in cryoprotectant at −20°C.
Brain sections were stored in cryoprotectant (300 ml ethylene glycol, 550 ml PB, 300 g Sucrose, volume to 1000 ml with H2O; pH7.2) until used.
Brain sections were stored in cryoprotectant solution (37.5% v/v ethylene glycol, 37.5% w/w sucrose, in PBS pH 7.4) at -20°C until processed for IHC.
Similar(57)
The brain was removed and sectioned coronally at 40 µm on a vibratome (VT1200, Leica Microsystems), and successive sections were stored in PBS containing 0.01% sodium azide at 4°C.
Sections were stored at −80°C until stained.
Sections were stored in PBS with azide.
Brains were sectioned frozen in the coronal plane at a thickness of 40 µm on a sliding microtome (Leica Microsystems Inc., Richmond Hill, ON) and 6 series of sections were stored in cryoprotectant (30% glycerol, 30% ethoxyethanol, 40% PBS).
Sections were stored at −80 °C until use.
Mounted sections were stored at −80°C.
For long-term storage, AP-stained brain sections were equilibrated in ethanol and stored at −20°C.
Brains were stored at 4°C. 75 µm-thick sagittal brain sections were made from transgenic 5HT3A-EGFP mice (P15) using a vibratome (Leica VT1000S).
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