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Brain sections were processed for CRH mRNA in situ hybridization.
One-in-three series of these brain sections were processed for immunostaining.
On 10th day animals were sacrificed and their brain sections were processed for AVT immunohistochemistry and their serum was analyzed for testosterone levels.
At 2 weeks post-transplantation, brain sections were processed for β-gal staining to identify the transplanted cells and injection tracts of the transplants were clearly visible and most of the LacZ-positive B10 cells migrated from the original injection sites and localized at the boundary of lesion cavity which was fused with dilated lateral ventricles (Figures 6B E).
Free-floating brain sections were processed for BrdU immunohistochemistry.
For immunohistochemistry (IHC) and immunofluorescence (IF), free-floating brain sections were processed as previously described [29].
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For architectonically identifying V1 and its laminar structure, one set of brain sections was processed for cytochrome oxidase (CO) (Wong-Riley 1979) and another set was stained for Nissl substance.
Brain cryostat sections were processed for in situ hybridization and immunohistochemidtry. Immunohistochemistry labelling was performed immediately after the last washing of the in situ hybridization procedure.
For each brain, four sections were processed for total binding and two other sections for nonspecific binding.
Brain tissue sections were processed when two to three adjacent sections showed no sign of demyelination with LFB staining and no perivascular cuffs of lymphocytes using hematoxylin and eosin staining (6, 15).
Furthermore, brain, liver and duodenum cryostat sections were processed for cholinesterase histochemistry using various substrates and inhibitors.
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brain sections were used
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brain sections were exposed
brain sections were performed
brain sections were immersed
brain sections were treated
brain sections were placed
brain regions were processed
brain sections were incubated
brain sections were deparaffinized
brain sections were stored
brain sections were obtained
brain sections were stained
brain images were processed
brain homogenates were processed
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