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After classical embedding in paraffin, 5 µm brain sections were prepared.
Brain sections were prepared from tau609, tau784, and non-Tg mice at 6 and 24 months.
Fixed brain sections were prepared during 10 00-14 00h as described above.
In each group, eight rats were examined in the Morris water maze to assess spatial learning and memory, six rats were sacrificed and brain sections were prepared for TUNEL staining and Bcl-2/caspase-3 immunohistochemistry, and six rats were sacrificed and tissue homogenates were prepared for Western blot assay of phospho-p38 MAPK expression.
Brain sections were prepared and examined quantitatively.
followed by transcardial infusion with paraformaldehyde (4% (w/v)), coronal brain sections were prepared as described in Abdel Baki, et al., 2009 [8].
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Rcan2−/− mice were anesthetized with Nembutal and transcardially perfused with 4% paraformaldehyde in 100 mM PBS for 10 min. Brains were dissected and 500 µm sections were prepared using brain matrices (Aster Industries, Pittsburgh, Pennsylvania, USA).
Three weeks after tumor inoculation, six mice from each group were sacrificed by cardiac perfusion with 4% formaldehyde in PBS, their brains were removed, and paraffin sections were prepared.
Fresh sections were prepared from brains that had been removed rapidly from the skulls of decapitated mice and harvested in the same manner as for electrophysiological experiments.
Frozen sections were prepared from each brain as described earlier and incubated with rabbit polyclonal anti-GFP Ab (1 : 1000) for 3 hours at room temperature (rt) and then with secondary antibodies for 1 hour at rt.
Brains were dissected and 20 µm cryostat sections were prepared.
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