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Approximately 5 brain sections were placed per insert, and media was changed every 3 days.
For the analyses of the injury response, 15 μm thick coronal brain sections were placed on charged microscope slides and stored at -20°C.
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Five lines per coronal brain section were placed starting from the ventricle and ending at the cortical surface.
The brain was cut into slices (30 μm thick) using a cryostat and the sections were placed on glass slides.
5 μm sections were placed on chemically charged slides.
The sections were placed in TBS contained Tween-20 (0.05%).
Leaf sections were placed into a glass vial for staining.
Sections were placed into 0.12 M PBS for 10 minutes.
For TEM, sections were placed onto formvar-carbon coated grids.
The sections were placed on Superfrost Plus glass slides (Electron Microscopy Sciences).
These sections were placed on silane-treated glass slides.
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