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Thereafter, the brain was frozen on dry ice, imbedded in OCT matrix (Thermo Fischer, Waltham, MA), and serial 20 μM frozen brain sections were obtained at −20 °C in either coronal or axial planes using OTF5000 cyromicrotome (Hacker Bright Instruments, Winnsboro, SC).
Brain sections were obtained in a microtome (Leica, Wetzlar, Germany) at 50 µm.
Brain sections were obtained and processed as described in [12], except for E17 embryo brains that were fixed by immersion in 4% paraformaldehyde.
Coronal brain sections were obtained, as described above.
Confocal images of immunofluorescently stained cortical neurons and rat brain sections were obtained with a Zeiss LSM710NLO microscope.
At 24 h after PT, the mice were killed and brain sections were obtained for histological evaluation of ischaemia-induced tissue damage.
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To assess areas of tumor colonization, photomicrographs of each brain section were obtained using ScanScope CS slide scanner (Aperio) and analyzed using ImageScope (Aperio).
Brain tissue sections were obtained for normal adult and fetal brains from BioChain (BioChain Institute, Inc., Hayward, CA, USA).
Fluorescence images of whole brain coronal sections were obtained using a SteREO Lumar.V12 fluorescence stereoscope (Carl Zeiss MicroImaging GmbH, Oberkochen Germany), whereas striatal images were captured using a Leica TCS 4D confocal scanning laser microscope (Leica Lasertechnik GmbH).
Paraffin-embedded brain tissue sections were obtained from different parts (temporal, frontal and occipital lobes, hippocampus, amygdala, and pre- and postcentral gyruses) of the brains of seven patients with AD, or from the corresponding parts of the normal brain (BioChain Institute, Newark, CA, USA).
Using these parameters, 20 whole brain contiguous axial sections were obtained angled parallel to the inter-commissural line.
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