Sentence examples for brain sections were immunostained from inspiring English sources

Brain sections were Immunostained as described [35].

De-waxed and re-hydrated 5 µm brain sections were immunostained for PrPsc using SAF84 (SPI Bio) and 2G11 (Pourquier) monoclonal antibodies with or without an additional step using streptomycin sulfate, following a procedure reported in detail elsewhere [72].

Paraffin-embedded sections (6 µm) of mice brain tissue were deparaffinized and hydrated followed by antigen retrieval with boiled 0.01 M citrate buffer, pH 6.0, for 10 min. Brain sections were immunostained with anti-GFAP, anti-Iba-1 or anti-LC3 antibodies.

For the assessment of number of activated microglia, brain sections were immunostained with anti-ionized calcium binding adaptor molecule-1.

After 21 days, brain sections were immunostained with anti-A β1-42 and caspase3 antibodies to validate the model.

5XFAD/apoEm/m and 5XFAD/apoE4/4 brain sections were immunostained with HJ6.3B for mouse apoE and HJ15.7B for human apoE4 (scale bar, 400 μm).

Another brain section was immunostained using goat anti-collagen primary antibody (1 : 100; 1 h at 4°C) (Southern Biotechnology Associates, Birmingham, AL, USA) and biotinylated anti-goat secondary antibody (1 : 500; 30 min at room temperature) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) to identify mature and immature brain vessels.

The last brain section was immunostained using mouse anti- α smooth muscle actin (α-SMA) primary antibody (1 : 500; 1 h at 4°C) (Dako, Glostrup, Denmark) and biotinylated anti-mouse secondary antibody (1 : 1000; 30 min at room temperature) (Valbiotech, Paris, France) to identify immature brain vessels.

Brain cortical sections were immunostained for CD11b, a marker of microglial cells (figure 3A).

3-month-old FeCγ25 APP−/− mice were injected with BrdU for 3 days, brains were harvested on the 4th day and sections were immunostained for BrdU (Fig. 4A, B) or DCX (Fig. 4C).

Brains were fixed at E17.5 and dorsomedial telencephalon sections were immunostained with antibodies against GFP and Ki67.