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Low magnification images of entire brain sections were generated using an Axioscan Z1 (Carl Zeiss, Göttingen, Germany) slide scanner.
Processed brains were frozen with dry ice-chilled isopentane, placed on dry ice, and 100 μm brain sections were generated on a cryostat.
Brains were removed, frozen over dry ice, and stored at −80°C. 25 μm fresh frozen brain sections were generated on a cryostat, were adhered to gelatin-coated slides, dried at room temperature, and stored at −80°C.
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Brains were dissected, and 1-mm-thick coronal sections were generated using an acrylic rat brain matrix (Electron Microscopy Sciences).
Optical sections were generated using ApoTome (Carl Zeiss).
Brain sections were prepared and examined quantitatively.
Brain sections were processed for CRH mRNA in situ hybridization.
Brain sections were examined for the hippocampal neuronal damage.
Brain sections were Immunostained as described [35].
Brain sections were stained for brain injury markers.
For immunohistochemistry, brain sections were briefly rehydrated in PBS.
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