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S1R-KO mouse brain sections were found to be devoid of S1R staining.
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To confirm this assumption, brain sections were stained for activated microglia which were found to be present prominently in 1 mg and 10 mg B[a]P treated animals and also to a lesser extent in 100 µg treated group.
Brain sections were prepared and examined quantitatively.
Brain sections were processed for CRH mRNA in situ hybridization.
Brain sections were examined for the hippocampal neuronal damage.
Brain sections were Immunostained as described [35].
Brain sections were stained for brain injury markers.
For immunohistochemistry, brain sections were briefly rehydrated in PBS.
The brain sections were stored in cryoprotectant at −20°C.
Brain sections were incubated overnight with primary antibodies at 4°C.
Serial brain sections were also labeled with S100a9 antibodies.
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