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For immunohistochemical staining, brain sections were deparaffinized in xylene and rehydrated in graded alcohols.
For the identification of possible amyloid-like structures, 10 µm-thick brain sections were deparaffinized, stained with 0.5% Congo red (Merck, Darmstadt, Germany) alcohol solution for 15 minutes, destained in 0.2% KOH, subsequently counterstained with Mayer's hematoxyline, and after a short dehydration, they were finally cleared in xylene.
Brain sections were deparaffinized and incubated with the quenching solution for 10 min.
The formalin-fixed paraffin-embedded brain sections were deparaffinized by standard method.
The brain sections were deparaffinized and non-specific endogenous peroxidase activity blocked with 3.0%H2O22 for 5-min at room temperature (RT).
Formalin fixed, paraffin embedded mouse brain sections were deparaffinized and antigen retrieval was performed for 1 minute in a pressure cooker with antigen unmasking solution (Vector Laboratories, Burlingame, CA).
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Streck-fixed, paraffin-embedded brain tissue sections were deparaffinized, rehydrated, and then post-fixed in Streck tissue fixative (Streck Laboratories, Omaha, NE) for 20 minutes.
The brain tissue sections were deparaffinized, rehydrated and washed as described previously (Mukherjee et al., 2004).
Paraffin sections were deparaffinized and rehydrated.
Briefly, tissue sections were deparaffinized and rehydrated.
Sections were deparaffinized and stained with HE.
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