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Exact(28)
Brain sections were cut sagitally at a thickness of 40 μm.
For conventional histological examination, 40-μm thick sagittal brain sections were cut on a vibratome (VT1200S, Leica) after perfusion and post-fixation in 40-μm thicknight.
Because in the first group, brain sections were cut sagitally to have an overview of the complete brain, we believe this data cannot be directly compared against the first cohort of animals, which was cut coronally to focus on the hippocampus, thalamus and amygdala.
Sagittal brain sections were cut at 30 µm in a cryostat and used for immunohistochemical analysis of EB leakage.
After perfusion, 40 µm brain sections were cut on a vibratome and processed with a standard immunohistochemical procedure using specific primary antibodies.
Brain sections were cut on a vibratome and transferred into ACSF which was continuously oxygenated with 95% O2 and 5% CO2.
Similar(32)
For each particular gene, 25 μm thick brain sections are cut at every 100 200 μm throughout the entire mouse brain.
Tissues were deep frozen in liquid nitrogen and serial 8 μm brain coronal frozen sections were cut for histological analyses.
Each brain was then placed in a brain matrix, and coronal sections were cut into 2 mm slices.
For each brain, 12 µm frozen sections were cut throughout the entire left hemisphere in the sagittal plane.
One hour after perfusion, the brain was removed and vibratome sections were cut at 60 μm tangential to the dorsal surface of the brain.
More suggestions(15)
brain sections were deparaffinized
brain hemispheres were cut
brain sections were stored
brain sections were stained
brain sections were collected
brain tissues were cut
brain sections were selected
brain sections were coverslipped
brain sections were used
brain sections were exposed
brain sections were performed
brain sections were mounted
brain sections were rinsed
brain sections were washed
brain sections were assayed
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