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Brain sections were coverslipped and incubated overnight at 55°C.
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All sections were coverslipped with Glycergel Mounting Medium (Dako, Denmark).
After washing with PBS, Vectashield (Vector Laboratories) mounting medium was applied and sections were coverslipped and imaged.
Immunostained sections were coverslipped using fluorsave (Calbiochem) as the mounting medium.
After the chromogenic reaction, the sections were coverslipped and observed under a microscope.
Finally, sections were coverslipped in Entellan mounting medium (BDH Chemicals, Toronto, ON, Canada).
After washing four times in PBS, the sections were coverslipped with SlowFade Antifade Kit (Invitrogen).
Finally, sections were coverslipped using DPX (Sigma).
All sections were coverslipped with Mowiol.
Sections were coverslipped using Vectashield DAPI mounting media (Vector Labs).
Sections were coverslipped and confocal microscopy was performed using an Olympus FluoView FV1000.
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