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To determine whether MVC treatment altered macrophage activation in the brain, sections were characterized by immunohistochemistry and digital image analysis.
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Brain sections were prepared and examined quantitatively.
Brain sections were processed for CRH mRNA in situ hybridization.
Brain sections were examined for the hippocampal neuronal damage.
Brain sections were Immunostained as described [35].
Brain sections were stained for brain injury markers.
For immunohistochemistry, brain sections were briefly rehydrated in PBS.
The brain sections were stored in cryoprotectant at −20°C.
Brain sections were prepared as above.
Brain sections were subsequently washed with d.d.
Mouse brain sections were prepared as described.
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