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Brain sections were blocked in 10% NGS and 1% Triton.
Control and PML brain sections were blocked with 5% normal horse serum in 0.1% PBS/BSA for 2 h at room temperature.
Brain sections were blocked with 10%% (v/v) normal goat serum and 0.3 % Triton X-100 for 1 h and incubated with primary antibody overnight as above.
Brain sections were blocked using 10%% normal animal serum (ScyTek, Logan, Utah, USA) in Dulbecco's phosphate buffered saline (DPBS; Sigma-Aldrich) for 20 min at RT.
Brain sections were blocked with 5% donkey serum, 1% bovine serum albumin, and 0.03% hydrogen peroxide in PBS and incubated with goat anti-Glut3 antibodies (I-14, Santa Cruz Biotechnology) or rabbit anti-GABAα1 antibodies (Alomone Labs) at 4°C for two days followed by incubation with biotinylated anti-goat or anti-rabbit IgG(H + L) at 4°C overnight.
Similar(55)
Sections were blocked for 10 min in UV blocking agent.
The sections were blocked by Chemmate Peroxidase Blocking Solution (DakoCytomation).
Sections were blocked with 1% BSA/PBS.
Sections were blocked using blocking solution (Protein Block Serum-Free; DAKO, Tokyo, Japan).
Brain sections were permeabilized and blocked in PBS, pH 7.4, containing 0.2% CHAPS and 2% BSA (Sigma-Aldrich), then incubated with primary antibodies diluted in PBS, pH 7.4, containing 0.1% CHAPS and 2% BSA, for 3 h at room temperature.
Brain sections were permeabilized and blocked in permeabilization buffer (3% BSA, 0.3% Triton X-100, and PBS [pH 7.4]) with rocking overnight at 4°C.
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