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Six brain sections were assayed for each gene category.
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The brain sections were analyzed by TUNEL assay (Roche) or stained with cresyl violet for measurement of neuronal loss.
After the completion of spaced active place avoidance training, rats were sacrificed, coronal brain sections were prepared and the loss of cortical tissue at the impact site was assayed.
For autoradiography, brain sections were brought to room temperature for 5 min and then pre-incubated in assay buffer (30 mM Tris-HCl, pH 7.4 + 0.1% BSA) for 10 min at RT.
Brain sections were prepared and examined quantitatively.
Brain sections were processed for CRH mRNA in situ hybridization.
Brain sections were examined for the hippocampal neuronal damage.
Brain sections were Immunostained as described [35].
Brain sections were stained for brain injury markers.
For immunohistochemistry, brain sections were briefly rehydrated in PBS.
The brain sections were stored in cryoprotectant at −20°C.
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