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Stained brain sections were analyzed using a Zeiss Axioplan 2 light microscope including a Sony DXC-930P color video camera system (Zeiss, Jena, Germany).
Brain sections were analyzed using a Zeiss Axio Observer inverted microscope equipped with a LSM 5 Exciter confocal scanning system (Carl Zeiss, Jena, Germany).
Brain sections were analyzed using a Zeiss confocal microscope.
The brain sections were analyzed by TUNEL assay (Roche) or stained with cresyl violet for measurement of neuronal loss.
From each brain at least 10 cryosections (each showing 3 brain sections) were analyzed, with at least 70 μm between individual sections analyzed.
Extent of neuronal damage in the brain sections were analyzed in a double-blind manner using the following criteria for the grading scale: 0: no observable neuronal damage; 1: damaged neurons populate 0%to25%5% of area; 2: damaged neurons populate 25%to50%0% of area; 3: damaged neurons populate 50%to75%5% of area; and 4: damaged neurons populate >75% of area (Cui et al., 2012; Hadass et al., 2013).
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Here, one quantitative approach to the measurement of β-amyloid plaques in brain sections was analyzed for sources of variability due to sampling.
Cellular expression of PLD4 mRNA in P7 and P21 mouse brain sections was analyzed by in situ hybridization (ISH) (Fig. 4).
The resulting tryptic peptides in a mouse brain section were analyzed by mass spectrometry imaging at 50 μm pixel size.
Brain tissue sections were analyzed using autoradiography and subsequent H/E staining.
Three days after the ischemic stroke, brain coronal sections were analyzed for infarct formation using TTC staining.
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