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Adjacent sets of unfixed rat brain sections were analysed in triplicate for quantitative autoradiography for TSPO with 125I]-CLINDE, OX-42 (microglial activation) immunohistochemistry and cresyl violet (cell loss).
For each animal, 5 comparable brain sections were analysed.
The animals were killed 7 days after injection, the brain sections were analysed by microscopy.
At the end of imaging protocol (24 h after injection), brain sections were analysed by fluorescent microscopy to examine localisation of injected Cy5.5-labelled sdAbs.
Brain sections were analysed under a light microscope and areas of neuronal cell death recorded.
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In order to verify the increased abundance of the glial fibrillary acidic protein (GFAP) in the Dp427-deficient brain, as well as study vascularization by labelling brain tissue with von Willebrand factor (vWF), mdx- 4cv versus wild type cortex sections were analysed by immunofluorescence microscopy [ 32].
Stained sections were analysed using TissueMap (Definiens, Munich, Germany).
Histologically stained paraffin wax embedded sections were analysed as previously described [18].
The sections were analysed for the presence or absence of aspirated material.
One to three sections were analysed for 4 animals per group (rapamycin vs vehicle).
Two sections were analysed for each case and from each composite image two fields were chosen for counting.
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