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Serial brain sections were also labeled with S100a9 antibodies.
These brain sections were also used in this study for possible herpesvirus infection detection.
Brain sections were also triple-labeled using anti-Iba-1 antibody for microglia, thioflavin S staining for A β deposition and MAP2 for neuronal dendrites.
Frozen AD brain sections were also stained by p-FTAA-MeOPh, and as mentioned earlier, Aβ deposits were easily identified due to strong emission from the probe.
Because tau APFs are best prepared from tau oligomers in vitro, DLB brain sections were also analyzed for the presence of tau oligomers colocalized with APFs by immunofluorescent double labeling with αAPF and T22, a polyclonal conformation-specific antibody against tau oligomers [ 40].
To determine whether the increase in vascular LAMP-1 immunoreactivity in the brains of α-Gal A deficient mice was also localized to endothelial cells, brain sections were also double-labeled with an anti-LAMP-1 antibody and fluorescent potato lectin (FPL), a fluorescein-tagged lectin that binds to cell-surface receptors on vascular endothelial cells [ 26].
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Sections were also stained by immunofluorescence.
Brain sections were prepared and examined quantitatively.
Brain sections were processed for CRH mRNA in situ hybridization.
Brain sections were examined for the hippocampal neuronal damage.
Brain sections were Immunostained as described [35].
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