Extensive GFP transgene expression was observed by fluorescence microscopy in serial brain tissue sections spanning the caudate and putamen (Supplementary Fig. 1) over an anterior posterior posterior distance of 12 mm.
Coronal sections spanning the brain were cut in a cryostat at a thickness of 16 µm in a Zeiss cryostat.
Next we examined the central brain volume of yata mutants by measuring the area of the paraffin sections spanning the entire brain.
"This web is constructed from a multitude of straight sections, tessellating [tiling] the air with polygons and line sections, spanning the depth of the space," said Woods.
Therefore, we examined individual SPBs using transmission EM, analyzing serial sections spanning the entire SPB.
These brains were sectioned in the coronal plane at 18 μm and sections spanning the rostral-caudal extent of the brain were processed with ISH.
We divided the box into 1×40-pixel sections, with each section spanning the width of the box.
We then used a range of sections spanning 5 sections above this section to 15 sections below this section (approximately 250 μm above the top of layer 4 to 800 μm below the top of layer 4).
At different time points, the rate of survival of IhNSC-Ps was evaluated by counting GFP+ or HuN+ cells in serial brain sections (each 200 µm apart) spanning the graft area of n = 3 rats per time point.
Inside, five sections span a brain-wide collision of worlds, including web porn, alchemy, art, America, action movies, fucking, S&M, Rainer Werner Fassbinder, Leonard Cohen, childbirth, money.
