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In situ hybridization analyses of embryonic and neonatal brain sections revealed that snx3 mRNA is mainly expressed in the cerebral cortex, hippocampus, piriform cortex, cerebellum, and spinal cord.
In situ hybridization analyses of brain sections revealed that transgene expression was restricted to the forebrain such as the hippocampus and neocortex in the adult brain (Fig. 1B).
Histological examination of brain sections revealed that mice displaying symptoms of ECM strongly expressed OX40 on a subset of accumulating lymphoid cells in and adjacent to blood vessels in the brain (Fig. 5A).
We focused on this period of development because immunostaining of cortical brain sections revealed that EphB2 is expressed in the neuropil of cortex at this time (Figure 1), with the highest level of expression found in the subgranular layers.
Given that these mice die after birth, embryos were analyzed at E18. Nissl-staining of brain sections revealed that the overall organization and cytoarchitecture of podxl brains was similar to that of wt embryos.
RFP immunohistochemistry on brain sections revealed that 4D mice displayed mosaic RFP expression, with approximately 30 40% of cells in the VZ being RFP+.
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Post hoc histological examination of brain sections revealed robust and bilateral expression of hM3Dq or hM4Di in CA3 pyramidal neurons (Fig. 8b).
Analysis of cortical brain sections revealed a very similar picture (Fig. 2).
In situ hybridization of the diseased brain sections revealed induction of miR-21 in neurons.
P7 developing mouse brain sections revealed expression in the anterior thalamic nuclei, and HPF.
Combining in situ hybridization and immunohistochemistry on single brain sections revealed only partly overlapping expression patterns (Fig. 2).
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