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No labelling is visible in brain sections obtained from calbindin D-28k knockout mice [46].
Brain sections obtained from perfused brains were mounted on slides and were stained with Cresyl Violet.
Total RNA was isolated from brain sections obtained from Mecp2-null (KO) mice and their wild type (WT) littermates.
In addition, we also show the presence of ubiquitin/ISG15 double-positive inclusions in the brain sections obtained from A-T patients (Fig. 7).
The absence of iNOS immunoreactivity in the ATP-injected brain was not due to experimental error since iNOS was readily detected in brain sections obtained from positive control rats systemically injected with LPS (data not shown).
Histological analysis of brain sections obtained from naïve or at day 5 post-infection of C57BL/6 did show very discrete erythrocyte aggregates observed in sections from olfactory bulbs and other anatomical parts of the brain (mouse 1, panel 4, Fig 3A).
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The presence of Lys63-linked polyubiquitin and ISG15 inclusions/aggregates in the mid-brain sections obtained from A-T patients further strengthens this possibility.
As shown in Fig. 7B, the dramatic increase in both ubiquitin/ISG15 double-positive inclusions (see arrows in merged images) was found in the mid-brain sections obtained from all three A-T patients tested.
Sixty µm brain sections, obtained on a cryostat, were stained and mounted in resinous medium.
Free-floating sections obtained from each brain were incubated with a monoclonal anti-GFAP-Cy3 conjugate antibody (1 : 500, Sigma-Aldrich, Madrid, Spain) 24 h at room temperature.
The considered tumor area was the largest one of all the sections obtained from each brain, and the infiltration length was measured from the implant site to the longest distance reached by astrocytoma cells.
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