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Twenty µm brain sections including the SCN and 5 µm ocular sections were prepared using a cryomicrotome (CM1900, Leica, Germany).
The presence and severity of TDP-43 immunoreactive structures were assessed in four brain sections including the anterior hippocampus, amygdala, medulla oblongata, and lumbar spinal cord.
No immunolabelling with mAb 2G11 occurred in brain sections, including the rostral colliculus, from an un-inoculated pig whereas immunolabelling of neuronal perikarya was evident with mAb L42 in the brain of this pig.
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Rat brains were rapidly isolated and the frozen brain sections included the core and shell subdivisions of the NAc were cut as described previously (Xiong et al, 2011).
One brain section including the hippocampal formation already quantified for the number of DiI-traced neurons was selected by chance and stained with aldehyde fuchsin-Darrow red.
The mute storytelling in the native tongue lit up several brain sections, including Broca's region, which is thought to store grammar knowledge.
The staining was performed on two brain sections including either external globus pallidus (GPe) or substantia nigra pars reticulata (SNr).
Finally, bFKB1 increased phospho-ERK signal in pancreas as expected, but not in various brain sections including circumventricular organs (Fig. S5D).
The examination of the viral load in organs taken on day three, post first immunization, revealed no infectious virus in the lung, brain, (three sections including the cerebrum, cerebellum, and pons), intestine, or kidney tissues.
Fig. 2 shows schematic drawing of frontal brain section including RVL region with different symbol (circle, triangle and star) indicating the labelled neurons presence.
Serial frontal 4 µm sections including the entire brain were prepared.
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