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Typical vacuolation of prion disease was observed in brain sections from animals inoculated with the Solid MBM Residue sc (Figure 5C and D).
Hamster brain sections from animals at the time of sacrifice showed increased immunoreactive GFAP-positive astrocytes following inoculation with PrPsc alone (day 75) or PrPsc+RADA (day 115) as compared to animals inoculated with PrPc+RADA (Fig. 5A; top panels).
To test whether 22C4 IgG and 22C4 scFv also bound to amyloid plaques and CAA in vivo, brain sections from animals that were treated intranasally for 14 weeks with either 22C4 scFv (30 µg twice a week), 22C4 IgG (20 µg once a week) or PBS (once a week) were stained with an anti-5xHis antibody or an anti-mouse Cy3 antibody to detect 22C4 scFv and 22C4 IgG, respectively (Fig 2 K to N).
WFA antibody was used to visualize perineuronal nets in brain sections from animals that were injected with 231BR cells.
As shown in Figure 5, MSC PEDF tumours demonstrated cellular apoptosis in tumour mass (C and E), whereas tumour-bearing brain sections from animals treated with MSC show negligible TUNEL staining of the tumour (D and F).
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Total number of LacZ-positve B10 cells in the brain sections from ICH animals was determined by stereological estimation.
We have also found less viral immunolabeling in the brain sections from EEPy animals compared to IEPy (Fig. S1).
Total number of LacZ-positive B10 cells in the brain sections from ICH animals was determined by stereological analysis at 2 weeks and 6-weeks post-transplantation.
Total number of human NuMA-positive F3 (n = 3) and F3.Akt1 (n = 3) hNSCs in the brain sections from ICH animals was determined by stereological estimation as described previously [16], [17].
Coronally cut 6 μm paraffin embedded formol fixed brain sections from terminal animals (~260 dpi) were de-waxed, microwaved in citrate buffer quenched with methyl peroxide and blocked using normal goat serum.
All brain sections from different animal groups were simultaneously run to ensure identical staining conditions.
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