Six weeks later, we immunolabeled the brain sections for mCherry.
We generated serial sets of histological brain sections for 145 reproducible forebrain enhancers, resulting in a publicly accessible web-based data collection comprising more than 32,000 sections.
Triple label immunofluorescence staining of brain sections for galanin, GnRH and the presynaptic vesicle marker synaptophysin coupled with confocal microscopy was employed to identify galanin synapses to GnRH perikarya.
To test whether Lgl1 protein expression is detectable in differentiated OL in situ, as suggested by our transcript analyses, we co-immunostained P35 wildtype mouse brain sections for Lgl1, OPC markers PDGFRα, and NG2 (Fig. 1d, Supplementary Fig. 1a), and committed OL marker CC1 (Fig. 1e 8.
To further examine the inflammatory response, we stained brain sections for the microglia marker Iba-1 (Fig. 6A).
For the in vivo experiment, manual cell counting was performed on 18 and 30 µm thick brain sections for two days and two weeks old animals, respectively.
Immunohistochemistry was performed on control, JEV and JEV+M brain sections for the markers of proliferating cells: Ki-67, and PCNA.
Although Namimatsu et al. [34] have employed the citraconic anhydride in several tissues, as far as we know nobody has tested this AR method in human brain sections for immunohistochemical free-floating studies.
To assess GLP-1R expression in the brain, we immunostained brain sections for GLP-1R.
To identify the cells containing p62 in Fig4 −/− brain, we co-immunostained brain sections for p62 and cell-specific markers.
