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Labelling was absent from QC knock-out mouse brain sections, demonstrating the specificity of the immunohistochemical QC detection (Fig. 1).
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P7 developing mouse brain sections demonstrated strong expression of the NOV MaxiPromoter reporter construct in the retromammillary nucleus (RM) and light expression in the HPF.
Twenty-four hours after administration of drug, autoradiography of brain sections demonstrated nuclear uptake of the radiopharmaceutical in cells throughout tumour while normal brain cells remained free of radioactivity.
Immunostaining of D1R-positive striatal neurons in brain sections demonstrated significant reduction of the striatum in TIF-IAD1RCre mice, indicating that depletion of TIF-IA caused striatal neurodegeneration and motor deficits.
Further analysis of the volume of each hemisphere based on brain sections demonstrated a significant decrease in the poststroke brain volume in GITR Ab mice, and a significant increase in that of GITR Fc mice, compared with control mice.
The immunodetection of Olig2+ cells in the same brain sections demonstrated that Olig2 was expressed by a minority of nuclei within the SVZ.
Immunohistochemical studies of another Kelch-related protein, Actinfilin, in rat brain sections demonstrated that it is broadly expressed in neurons of most regions of the brain, suggesting that Actinfilin may be a key player in the actin-based neuronal function [59].
Morphometric analysis of brain sections demonstrated that toluene exposure resulted in smaller brains together with an increase in the size of the ventricular system and a reduction in the size of the caudate nucleus.
Analysis with this antibody using age-matched control or AD brain sections demonstrated no staining in control brains while widespread labeling of NFTs, neuropil threads, and dystrophic neurites was observed in AD sections.
(G ) AChE histochemistry on fresh frozen brain sections demonstrates a significant reduction of striatal AChE in Dlx-CKO mice (t-test; t(22) = 5.16; p < 0.0001).
The peripheral gene expression studies reviewed in this section demonstrate the feasibility of peripherally extracted coding and noncoding RNA to provide insights into brain-based disorders.
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