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However, there is need to culture whole brain sections, containing the complex functional architecture.
Sequential coronal brain sections containing the LA at −3.36 Bregma (see below) were prepared on a vibratome at 40 µm.
To assess JNK activity in the nigrostriatal system of mice, we stained wildtype and Pink1−/− brain sections containing the substantia nigra with an antibody to phosphorylated c-Jun using the nickel-enhanced DAB method.
Because the size of the habenula significantly varied across anterior-posterior axis, we collected brain sections containing the habenula at the level where the most anterior tip of the ventral part of the third ventricles emerged immediately posterior to the central nucleus of the amygdala.
A vibrating tissue slicer was used to prepare 400 μm thick coronal brain sections containing the NAc core.
Coronal brain sections containing the ACC, STR, and primary somatosensory cortex (SSC) of adult mice were immunostained for GAD67.
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(D ) Fluorescence image of a coronal brain section containing the SCN from a CamiCre +; tdTom + mouse.
Three measures were used to quantify CNS-1 tumor growth: the number of brain sections containing cells, the total number of tumor-containing pixels in the sections, and the maximum width that the cells spread perpendicular to the initial injection track.
AP-labeled cells and immunolabeled cells or axons were manually counted in regions of interest in at least three sections per mouse and three to four mice per condition in coronal brain sections containing horizontal fibers of the anterior commissure.
R.J.E. and T.P.K. performed the SCN slice experiments under the direction of M.H.H. R.Z. performed the labeling of brain sections containing 3 with 6 and performed microscopy.
Paraffin-embedded post-mortem 4- μm-thick brain sections containing caudate-putamen were provided by the Tissue Bank at Hospital Universitario Fundación Alcorcón (Madrid, Spain), and were obtained and handled following the ethical guidelines of that institution.
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