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By indirect immunofluorescence (IF) analysis using an anti-GFP antibody, we quantified (i) the percentage of GFP+ cells (based on GFP expression in cells with defined nuclei) on serial coronal brain sections comprising the injection site and (ii) the GFP+ enclosed volume in the injected hemisphere at different time post-injection (PND10, PND21 and PND40).
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Furthermore, the section comprises the Sava River Bridge, spanning 1072 m.
For each brain, serial sections (20 μm thick) comprising the anterior border of the OVLT through the caudal aspect of the ARC (as defined in Experiment 1) were cut and slide mounted using a cryostat.
The final sections comprise a summary and conclusions.
Three sequential sections per animal comprising the OVLT through the midlevel region of the AVPV (corresponding to 0.24 through -0.12 mm from Bregma) were selected for analysis using a brain atlas for reference [ 52].
Complementing the ROI analysis, the whole-brain investigation comprised the following multiple regression analyses.
- Section 1 comprises the introduction.
Section 3 details the steps comprising the above procedures.
Brain sections were examined for the hippocampal neuronal damage.
Diffusely strong Survivin staining of sections from normal brain was observed in the ependymal cells lining the ventricular system, and choroidal epithelial cells comprising the CP.
Autoradiogram of brain section images for the different treatment groups.
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CEO of Professional Science Editing for Scientists @ prosciediting.com