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The quantification of expression was performed on a fixed surface from different brain sections calculating the mean intensity of pixels in dark field gray-scale images.
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In each brain section, we calculated the percentage of hemispheric area that was infarcted by dividing the infarct area by the area of the contralateral hemisphere *100.
In each brain section, we calculated the percentage of hemispheric area that was infarcted by dividing the infarct area by the area of the contralateral hemisphere *100 (See Text S1).
The areas of brain sections were calculated using the ImageJ software (NIH).
b Staining differences between WT and S1R-KO brain sections were calculated by comparing pixel intensities of regions of interest after normalizing the sections to their background reading.
We integrated rostral and caudal infarct areas across all seven sections from each brain to calculate the infarct volume.
Digital photographs were taken of brain sections and the ratio comparing the area of the infarct to the area of the ipsilateral hemisphere was calculated.
Brain sections without the primary antibodies served as negative controls.
Stereotaxic coordinates for the NB were calculated using brain sections from an average-sized adult female ferret (700 g).
Due to the variability in the glucose levels (as discussed in the "Blood glucose level" section), the data analysis was performed by calculating the ratio of various ROIs to the whole brain average.
The anatomical positioning of brain sections was determined using the mouse brain atlas [76].
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