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Abundant immunoreactive IBA1-positive microglia were detected in 75 d PrPsc and 115 d PrPsc+RADA brain sections as compared to minimal detection in sections from animals inoculated with PrPc+RADA (Fig. 5B; bottom panels).
Importantly, we note that there is a very high level of auto-fluorescence in brain sections as compared with other tissues, which is perhaps why this fluorescent autophagy reporter has not been used extensively to study autophagy in the brain in prior studies (despite widespread use in other tissues).
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The results demonstrated significant DNA fragmentation in pCU-treated brain tissue sections as compared to control sections (Figs. 6A B).
In contrast, expression of Bax was increased in pCU-treated brain tissue sections as compared to controls (Figs. 6C D).
No overexpression of AMPARs was observed in these tumor sections, as compared to normal brain tissues cerebellum and hippocampus, in which more abundant and intensive staining was observed (Figure 2A, a d).
Tumor volume (calculated along serial histologic brain sections as described in Materials and Methods) displayed a significant reduction of the tumor mass formed by Daoy cells in GlaB-treated mice compared to the control (Fig 7A and B).
We observed that uPAR and phospho FAK (Y397) staining were high in the irradiated brain sections compared to control brain sections (Fig. S3).
These have whiskers that are oval in cross section, as compared to the circular cross section of terrestrial mammals [33].
The use of 31 P-AC significantly increases brain activity as compared with NoBone-AC and overestimates brain activity as compared with CT-AC (p < 0.001).
FAK was highly expressed in the control tumor brains as compared to hUCBSC-treated brain sections, which showed high expression of CD81.
In the present single centre retrospective series, we have shown that using a standardised procedure and careful on-demand intraoperative frozen-section analysis can improve the diagnostic yield of stereotactic brain biopsy procedures as compared to a historical series.
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