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The areas of the vacuoles in the cell body and neuropil regions on each brain section were measured using NIH Image J software (http://rsb.info.nih.gov/ij/index.html).html
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For normalization of the densitometric measurements, the unspecific background staining of the brain sections was measured at cortical areas where no BrdU+ or Tbr1+ cells depending on the kind of immunostaining were detectable.
Three sections were measured for each mouse.
Positive areas measured in similar striatal surfaces from each brain section were averaged to obtain one value per animal.
For analysis of axonal spheroid formation in commissural axons, the sizes of 100 largest swellings from each section (one section from one brain) were measured and a total of 3 brains were examined for each group.
The brain section is outlined with a dashed line.
Areas of brain regions were measured from images of NISSL-stained sections using Zen software (Zeiss).
To investigate whether the NP42T peptide was able to prevent structural changes observed in this HD model, mice brain sections were prepared and lateral ventricle area measured.
Frozen brain sections were then prepared and processed for quantitative autoradiography using [125I]α-bungarotoxin to measure the effect of this treatment on low affinity nicotinic receptors.
Brain sections were prepared and examined quantitatively.
Brain sections were processed for CRH mRNA in situ hybridization.
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