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In order to confirm the results of the staining, a normal mouse brain section was used as a positive control.
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Brain sections were used for ex vivo autoradiography, fluorescence microscopy, Nissl staining, immunohistochemistry, and in situ hybridization.
(F) Brains dissected from mice injected with Evans blue intraperitoneally; (G) After perfusion, brain sections were used to examine Evans blue directly under fluorescent microscope.
(E) Brains dissected from mice injected with Evans blue intraperitoneally immediately after tail vein injection of mannitol; (F) After perfusion, brain sections were used to examine Evans blue directly under fluorescent microscope.
Because pooled brain sections were used for analysis, we cannot be confident that this small GM2 reduction is mediated by genistein.
Stained brain sections were used to identify and delineate the ROIs while optical densities were determined on the corresponding autoradiograms, since both types of images were superimposed.
Briefly, glioma cells co-cultured with hUCBSC or transfected with either siXIAP or scrambled vector (SV) or brain sections were used for this study.
Stroke mouse brain sections were used as a positive control.
Free-floating brain sections were used for immunohistochemical staining.
For measurement of autofluorescence, 5-μm-thick nondeparaffinized, unstained brain sections were used.
Serial brain sections were used to perform in situ hybridizations as previously described in [ 34].
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