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The samples were washed with 2-propanol and then washed with H2O.
Afterwards, the samples were washed to remove the unbound polymer.
Subsequently, samples were washed with deionized water at pH 8.
Afterwards, the samples were washed several times with distilled water.
Then, the samples were washed with DI water.
The samples were washed thoroughly with water to remove the dirt, the washed samples were then sun dried and pulverized.
The samples were washed with SB18T.
Then the samples were washed four times.
Samples were washed and reversibly cross linked.
After 30 min, the samples were washed with wash buffer, placed on a rocker for 10 min, and washed again.
Samples were washed three times with 400 µL washing buffer (see manual Streptavidin Mutein Matrix).
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