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In addition, necropsies were performed on 22 fetuses and 5 newborn red deer fawns; brain samples were tested for SBV genomic RNA and cellular β-actin transcripts by reverse transcription quantitative PCR (3 ).
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If an infection with SBV was suspected, based on arthrogryposis and hydranencephaly, a brain sample was tested by RT-PCR for SBV, while the competent authorities would trace the mother of the affected offspring to take a blood sample for serological testing.
‡If available, brain samples were used; otherwise crop samples or biopsy samples were tested.
Samples were tested twice.
Plasma samples were tested individually.
All samples were tested in triplicate throughout.
All experimental samples were tested in duplicate.
All patient samples were tested in duplicate.
Triplicate samples were tested.
assaying laboratory of the U.S. Atomic Energy Commission, where all samples are tested.
If the A sample tests positive, the B sample is tested later.
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