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The control brain samples were set at 1.0 with glyceraldehydes-3-phosphate dehydrogenase (GAPDH) pre-developed TaqMan assay reagent as endogenous control (FAM™ Dye/MGB probe).
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For calibration, the number of selected brain samples was set equal to the number of samples found in the other tissue classes.
All samples were set up in replicates.
The samples were set up in duplicate.
‡If available, brain samples were used; otherwise crop samples or biopsy samples were tested.
Lines of diluted solutions of brain samples were examined to determine amplification efficiencies for each primer set.
Blood and brain samples were taken in two sets of experiments after CLP to see the early (24 h) and late (10 days) effects of treatment.
Blood and brain samples were taken in two sets of experiments to see the early (24 h) and late (10 days) effects of treatment.
The brain samples were pulverized with a small mortar and pestle set over dry ice to a fine powder.
For Western-blot analysis, the brain samples were pulverized with a small mortar and pestle set over dry ice to a fine powder.
Second, a sub-set of s brain samples were randomly selected and the remaining non-selected brain samples were subsequently excluded from the data set.
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