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At least four different brain samples were quantified for each genotype.
Aβ1 40 and Aβ1 42 in human brain samples were quantified using ELISA kits from Invitrogen (Aβ40 ELISA KHB3481; Aβ1–42 ELISA KHB3442) as we have previously described (Vagnoni et al., 2012).
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Moreover, the concentrations of vitamin A in brain homogenate samples were quantified according to the previously described methods [23 25].
Total RNA was isolated from snap-frozen mouse brain tissues using a mirVANA miRNA isolation kit (Ambion), and RNA samples were quantified and evaluated for purity (260/280 nm ratio) using a spectrophotometer (BioPhotometer, Eppendorf).
RNA samples were quantified using spectrophotometry.
RNA samples were quantified using Multiskan Spectrum (Thermo, Electron Corporation) prior to use.
Bands from tumor samples were quantified using NIH1.6 Image software.
Protein samples were quantified using the BioRad Protein assay.
Samples were quantified in triplicate.
Samples were quantified using a Bradford assay.
Samples were quantified using peak area ratios.
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