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Brain samples were processed as described in detail before51.
All the mice were sacrificed on day 14 and brain samples were processed for neurochemical, biochemical and immunohistochemistry studies.
A total of 23 brain samples were processed for paraffin embedding and sectioning: 6 controls, 5 mild, 6 moderate and 6 severe hydrocephalic brains.
Each brain samples were processed separately for total RNA isolation using Trizol reagent, treated with DNase I and purified with RNA clean-up kit (Clontech, Mountain View, CA).
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Brain tissue samples were processed according to a standard BDNF sample preparation protocol (Promega, USA).
Samples were processed separately for each brain region with data generated in 2 batches for each cohort of animals.
All samples were processed individually.
Samples were processed within 1 h.
Samples were processed and analyzed by Cumberland Valley Analytical Services , Inc
Samples were processed and analysed by T.M.C. Correspondence to Tim M. Conway.
ERH samples were processed as described in Materials and Methods.
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