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Plasma and brain samples were prepared by precipitation with n-propanol.
Brain samples were prepared as 20% homogenates in 5% (w/v) glucose prior to dilution (1/100) in EIA buffer.
The mouse brain samples were prepared using the established techniques described in the literature [ 17].
Brain samples were prepared for electron microscopy as previously outlined in detail [ 34].
Fresh frozen autopsied human brain samples were prepared as 0.8 1.0 cm fragments for microarray gene expression analysis from frontal cortex dissections, which were validated by quantitative real-time polymerase chain reaction (Torres-Munoz JE, unpublished data, 2006).
Frozen mouse brain samples were prepared as 10% (wt/vol) homogenates in 5% glucose in distilled water in grinding tubes (Bio-Rad, Hercules, CA, USA) by using a TeSeE Precess 48 homogenizer (Bio-Rad) following the manufacturer's instructions.
Similar(50)
For virus isolation, a 10% suspension of brain sample was prepared and injected into flasks of RK13, equine dermal, and Vero-MARU cells (Vero M).
Brain DNA samples were prepared by lysing in Laird's lysis buffer plus proteinase K overnight followed by ethanol precipitation.
To separate CTFs and NTFs of ApoE, new samples were prepared from brains with the greatest proportion of fragments for both ApoE3 and ApoE4.
Samples were prepared from dissected brain regions and injected onto a C-18 80×4.6 mm column (ESA Inc., Chelmsford, MA, USA) with detection using a 2-channel Coulochem II electrochemical detector (ES Inc .. Data were processed on an EZChrome Elite Client Workstation (ESA Inc).
RNA samples were prepared from human brain tumors using TRIZOL (Invitrogen, Carlsbad, CA, USA).
More suggestions(16)
brain samples were isolated
brain samples were deproteinized
brain cells were prepared
brain samples were quantified
brain samples were measured
brain cortices were prepared
brain samples were included
brain pericytes were prepared
brain samples were weighed
brain samples were used
brain extracts were prepared
brain samples were shipped
brain sections were prepared
brain lysates were prepared
brain samples were performed
brain oligodendrocytes were prepared
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