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Brain samples were not diluted as concentrations were too low to quantify after dilution in some samples.
When brain samples were not treated with PK, characteristic protein bands representing porcine PrPc were obtained in all tested pigs regardless of post-mortem TSE status using mAb 6H4 and mAb 12B2 (lanes 2-7, Figure 4A and 4C), but not with mAb P4 (lanes 2-7, Figure 4B).
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Since our study using mice showed no apparent effect for antipsychotic administration on the expression levels of Fabp3 (Supplementary Material, Fig. S17A), Fabp5 (Supplementary Material, Fig. S17B) or Fabp7 (26) in the frontal cortex, it is probable that the differences in FABP5 expression between brain and lymphocyte samples were not due to the effects of antipsychotics.
Unlike rapidly activated monoaminergic activity that occurs following the initial pairing, most brain areas sampled were not affected when animals were re-introduced, regardless of visual signal reversal or length of separation between interactions.
Brain samples were collected after 1 week.
†No blood or brain samples were available.
Brain samples were shipped on dry ice.
Brain samples were homogenized in lysis buffer.
The especially high levels of magnetite found in the study's brain samples are shocking, he says, though not necessarily surprising given that they lived and worked in industrial environments with lots of air pollution.
Because human brain tissues are not readily available, often brains are divided among researchers, and small brain samples are available for individual studies.
For each study, several brain samples are needed from each group--diseased and healthy.
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