The radioactivity in 50 µl of serum and of weighed brain samples were measured by a dual channel γ-counter.
Amino acids (glutamine, glycine, glutamate, D-serine, L-serine) in rat brain samples were measured by column-switching HPLC (Shimadzu Co., Ltd., Kyoto, Japan) as reported previously [20].
Groups of mice were killed at 5 and 30 min p.i. Radioactivity of weighted brain samples was measured in a γ-counter (Wallac 1480-011 Automatic Gamma Counter, PerkinElmer, Waltham, MA, USA).
The concentration of Aβ40 and Aβ42 in cortical and hippocampal rat brain samples was measured using a sandwich ELISA method.
Dopamine and its major metabolite DOPAC in rat brain sample were measured by high-performance liquid chromatography (HPLC) coupled with electrochemical detection (Eicom Co., Ltd., Kyoto, Japan) as reported previously [19].
Level of vitamin C and vitamin E in brain homogenate samples were measured using detection kits according to the manufacturers' introduction (Catal. No. A009, A008, Nanjing Jiangcheng Bioengineering Institute, Nanjing, China).
Additionally, the activities of glutathione (GSH) and glutathione peroxidase (GSH-x) in brain homogenate samples were measured according to the manufacturers' introductions of the assay kits (Catal. No. A006-2, A005, Nanjing Jiancheng Bioengineering Institute, China).
The masses of the blood, heart, lung, liver, spleen, small intestine, kidney, brain and urine samples were measured, and activity was also measured using a well counter.
178 samples were measured.
The samples were measured in triplicate.
The samples were measured in aluminium pans.
