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Brain samples were isolated from mice on day 6 post-infection.
Brain samples were isolated from F1 progeny of reciprocal crosses at P10 (except in the case of the imprinting timecourse experiment) and total RNA was prepared.
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Total RNA from each brain sample was isolated using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), and RNA was quantified by OD 260.
First, RNA samples were isolated from brain tissues of whitefish and omul (one sample for each species).
RNA samples were isolated using Trizol (Invitrogen).
Samples were isolated from day 8 cultures.
RNA from cell cultures, adult hamster brain, PNEC and from fresh surgical tissue samples was isolated by guanidine isothiocyanate/cesium chloride ultracentrifugation [ 29] or by an Absolutely RNA kit (Stratagene, La Jolla, CA).
Total RNA of brains and imaginal discs from 10 third instar larvae per sample was isolated using TRIzol Lifee Technologies, Carlsbad, California, USA) chloroform extraction and isopropanol precipitation.
Eight of 209 brain samples were confirmed rabies positive using FAT (3.8%), and RV was successfully isolated by MIT from the samples.
Brain samples were obtained from cattle experimentally inoculated (IC) with various isolates of classical and atypical BSE.
Brain samples were collected after 1 week.
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