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Proteins with an altered expression in mdx- 4cv brain samples were grouped based on their protein class using the PANTHER database of protein families.
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For this analysis, samples were grouped into 6 groups: brain parenchyma (GFP−), brain vascular (GFP+), brain endothelial (GFP+PDGFRbeta−), postnatal brain GFP+, liver endothelial and lung endothelial.
For normalization, the samples were grouped according to brain areas.
Direct comparison of one-day-old and forager honey bees was obtained using four microarrays (two microarrays per dye-labeling assignment) per colony for a total of 12 microarrays per study in Group 1. Individual brain samples were used in all Group 2 studies, each study including 20 microarrays in a loop design.
All brain samples were matched by age (all groups p>0.05, t-test), post mortem delay (all groups p>0.05, t-test), gender (in total 13 males and 12 females and every group consisted of 2 or 3 males), MAPT haplotype (p>0.05, Fischer exact test) and ApoE-ε haplotype (p>0.05, Fischer exact test).
Rats were divided into five groups as following; Group 0 is composed of six rats which represent the control group from where brain samples were taken immediately after the sacrifice (at the 0-time interval), each of the remaining four studied groups is composed of eighteen rats.
Each age group had six rats, from which brain samples were taken, flash frozen, and stored at −80 °C.
The brain samples were deproteinized by acetonitrile with IS.
Embryonic brain samples were fixed overnight in 4% paraformaldehyde (PFA).
Blood and brain samples were never taken for research.
Brain samples were homogenized using a Virsonic 100 ultrasonic homogenizer.
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