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Blood and brain samples were frozen at −80°C with 10% glycerol until use.
Alternatively, brain samples were frozen at −80°C until later use for immunoblotting.
The brain samples were frozen in embedding medium (O.C.T.; Sakura Finetek, Torrance, CA, U.S.A). on a solid CO2/ethanol slush.
Similar(57)
Brain tissue samples were frozen and stored at −80 °C.
Brain tissue samples were frozen immediately in liquid nitrogen and stored at −80°C.
Immediately after sacrificing, liver, kidney, and brain tissue samples were frozen in liquid nitrogen and stored at −80 °C until further use.
Plasma and brain cerebrospinal fluid samples were frozen at −80°C until analysed.
Samples were frozen immediately until use.
Plasma samples were frozen at −70°C.
Samples were frozen at -80°C.
The water samples were frozen.
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